http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005164360-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_96eddd31165955882708d73c89118259 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-46 |
filingDate | 2005-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b25d8f9b1a11589f37c4c99f75ef4982 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_499f57b7f733065ba371d37ab4acd4af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f1910662fe28152f3914918ca76c5e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a78a687f410846f5d3e07c346e65b24 |
publicationDate | 2005-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2005164360-A1 |
titleOfInvention | Actinobacillus succinogenes shuttle vector and methods of use |
abstract | An Actinobacillus succinogenes plasmid vector which provides a means to overexpress proteins in A. succinogenes. The plasmid can be transformed efficiently by electroporation, and replicates in a stable manner in A. succinogenes. The plasmid comprises at least one marker gene, operably linked to a first promoter functional in Actinobacillus succinogenes, an origin of replication functional in Actinobacillus succinogenes, a second promoter isolated from Actinobacillus succinogenes, and a cloning site downstream from the second promoter. Plasmids pLGZ901, pLGZ920, pLGZ921, and pLGZ922 are disclosed. The pckA gene polypeptide sequence and nucleic acid sequence of Actinobacillus succinogenes, including the promoter and ribosome binding site, is disclosed. Furthermore, a method for producing a recombinant Actinobacillus succinogenes is described, including a method of transformation. Additionally, a recombinant Actinobacillus succinogenes is disclosed and a method for producing succinate utilizing this recombinant Actinobacillus succinogenes is described. |
priorityDate | 2003-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 246.