| abstract |
Provided are a real-time PCR monitoring apparatus and method. The real-time PCR monitoring apparatus includes a microchip-type PCR tube that has a PCR solution-containing PCR chamber, a micro-heater that applies heat to the PCR chamber of the PCR tube, a detection unit that detects a PCR product signal based on the amount of a PCR product of the PCR solution, a plurality of modules, each of which includes a cooling fan for lowering the inside air temperature and a control unit for adjusting the temperature of the PCR chamber of the PCR tube by controlling the micro-heater and the cooling fan, and receives the PCR tube, the micro-heater, and the detection unit, a base instrument that includes a power supply unit electrically connected to the modules for power supply and a data communication unit electrically connected to the modules for data communication with the control unit of each of the modules, and a display unit that displays data received from the data communication unit, wherein the control unit of each of the modules independently controls at least one of both the detection unit and the temperature of the PCR chamber of the PCR tube received in each of the modules. Therefore, co-amplification of different samples at different temperature conditions can be carried out and monitored in real time. |