| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc905cbef116a1ec61d12125af9427f3 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate |
2004-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01c32f4d7f17514350ea5bbee9908ae1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdd4254b08962c61e23f4814d3dca62b |
| publicationDate |
2005-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2005112603-A1 |
| titleOfInvention |
Method for purification, modification and immobilization of recombinant protein |
| abstract |
The present invention relates to a method for purifying, modifying and immobilizing recombinant protein. The method utilizes genetic engineering to tag a DNA sequence encoding a target protein with a specific tag and express the vector to obtain a recombinant protein. The recombinant protein is then purified and modified by an affinity column and modification reagent. After exchanging the recombinant protein with a decoupling reagent, the recombinant protein is immobilized onto a specific substrate. The method, combining steps of purification, modification and immobilization, provides convenience to using recombinant protein. The omission of the use of dialysis or molecular sieve leads to a shorter period dedicating for removing excessive reagents and the increase of efficiency of recombinant protein recovery. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010064861-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010064861-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2009034204-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8283125-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101160381-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100973428-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010256301-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11155575-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/ES-2319061-A1 |
| priorityDate |
2003-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |