http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005084878-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07c9542186133097bd6bbb7d92393090 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-08 |
| filingDate | 2004-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d86da25c94dd09a83fea219a1000182b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97fdff7b4ed04fb3e5fd30a59de1c68f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b2946d0c5d7002529120a795f8c3e7dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5188ef48c2fdd7635a04062bd3e688f4 |
| publicationDate | 2005-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2005084878-A1 |
| titleOfInvention | Method for isolating and cloning high molecular weight polynucleotide molecules from the environment |
| abstract | Methods and reagents for isolating polynucleotide molecules from uncultured microbial cells in an environmental sample, the method comprising: obtaining uncultured microbial cells from the sample, dispersing the cells from each other and from other components in the sample, purifying the dispersed cells via discontinuous gradient centrifugation wherein the cells are collected in an interface of the gradient, embedding the cells in agarose gel, to produce agarose gel blocks containing the cells, and lysing the cells within the agarose gel blocks, thereby releasing high molecular weight polynucleotide molecules from the cells. Also disclosed are methods and compositions for cloning the polynucleotide molecules so isolated, further comprising incubating the gel blocks with at least a suitable restriction endonuclease to partially digest the polynucleotide molecules, separating the digested polynucleotide molecules by pulse field electrophoresis, to recover fragments of polynucleotide molecules with size of at least 50 kb, ligating recovered fragments of polynucleotide molecules to a suitable cloning vector, and transforming a suitable host cell with the cloning vector containing the fragments of the high molecular weight polynucleotide molecules. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011031127-A1 |
| priorityDate | 2003-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 143.