| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2503-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5044
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-025
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-567
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate |
2004-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4604251f50471c86bffe51afdd35cbd0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_736261e300ee735018d4ba16b883023a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a73af8cebeacb252b0546786ae7f482
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_728bb295cb46c326a8e9cfe014376b1e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_534ec727191f84bc365278eb58a3d8a3 |
| publicationDate |
2005-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2005059094-A1 |
| titleOfInvention |
Electrophysiological assay using oocytes that express human ENaC and the use phenamil to improve the effect of ENaC enhancers in assays using membrane potential reporting dyes |
| abstract |
In one aspect, the present invention relates to a mammalian cell-based high-throughput assay for the profiling and screening of human epithelial sodium channel (hENaC) cloned from a human kidney c-DNA library and is also expressed in other tissues including human taste tissue. The present invention further relates to amphibian oocyte-based medium-throughput electrophysiological assays for identifying human ENaC modulators, preferably ENaC enhancers. Compounds that modulate ENaC function in a cell-based ENaC assay are expected to affect salty taste in humans. The assays described herein have advantages over existing cellular expression systems. In the case of mammalian cells, such assays can be run in standard 96 or 384 well culture plates in high-throughput mode with enhanced assay results being achieved by the use of a compound that inhibits ENaC function, preferably an amiloride derivative such as Phenamil. In the case of the inventive oocyte electrophysiological assays (two-electrode voltage-clamp technique), these assays facilitate the identification of compounds which specifically modulate human ENaC. The assays of the invention provide a robust screen useful to detect compounds that facilitate (enhance) or inhibit hENaC function. Compounds that enhance or block human ENaC channel activity should thereby modulate salty taste in humans. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007092185-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010311610-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9534035-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8105792-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010298167-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10048274-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7892756-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010009385-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008108148-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9459259-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009123942-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007092185-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10078087-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011236313-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10215759-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2557087-A1 |
| priorityDate |
2001-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |