| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91188 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y206-01016
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2003-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdaed4d4829296c1c1845cddfd7ea525
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_414b546ee66275cdb9d38fe1a366612d |
| publicationDate |
2005-02-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2005032145-A1 |
| titleOfInvention |
Methods for measuring activity of glutamine:fructose 6-phosphate amidotransferase and activity of inhibitors thereof |
| abstract |
The present invention pertains to methods for measuring the activity of glutamine:fructose 6-phosphate amidotransferase which comprise the steps of (a) forming a mixture of glutamine:fructose 6-phosphate amidotransferase, fructose 6-phosphate, and glutamine; (b) incubating the mixture under physiological conditions to allow the formation of glucosamine 6-phosphate; (c) acetylating the glucosamine 6-phosphate to form N-acetylglucosamine 6-phosphate; (d) reacting the N-acetylglucosamine 6-phosphate with Ehrlich's reagent; and (e) measuring the amount of N-acetylglucosamine 6-phosphate by determining the optical density at 500-610 nm of the mixture and comparing the amount of N-acetylglucosamine 6-phosphate with control samples. The present invention also pertains to methods for measuring the inhibitory activity of a test compound on glutamine:fructose 6-phosphate amidotransferase. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8524444-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8993718-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9573911-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8957075-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009325944-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010290987-A1 |
| priorityDate |
2002-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |