http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005019920-A1
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-005 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
filingDate | 2004-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_189e0793a5f4d1c441c49180cd32f680 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fe5b93404e465a23c7248c0b4577f52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2994adfa5f8434bb41b2ab7a588304f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f77d09749a396d30416350e6762689e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8a3f1213b4579be8ca62f86d4b34135 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9cc5a57ef6bb54a70d30e82e09019fad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a96cc040ab32b228857d6deca0ff3078 |
publicationDate | 2005-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2005019920-A1 |
titleOfInvention | Methods and compositions for rapid in vitro propagation of swertia chirata |
abstract | The present disclosure relates to methods and compositions for in vitro cultivation of species of Swertia , e.g. Swertia chirata (Ham.). The disclosure provides culture media comprising Murashige and Skoog (MS) basal culture medium, plant hormones preferably selected from the group consisting of benzyladenine (BAP), gibberellic acid (GA 3 ), and auxins, and other additives, e.g. sucrose and agar. Preferably, auxins are selected from the group consisting of indole acetic acid (IAA), indole butyric acid (IBA), and naphthalene acetic acid (NAA). Individual plant hormone concentrations are preferably from about 0.5 mg/L to about 5.0 mg/L. The disclosure provides methods of in vitro cultivation of Swertia chirata comprising contacting preferably axillary bud and/or shoot apex explants with an initiation medium comprising a modified MS basal culture medium, BAP, IAA, IBA, and NAA to produce a primary explant, contacting the primary explant with a shoot propagation medium comprising, a modified MS basal culture medium, BAP, GA 3 , and IAA to produce a secondary explant, contacting a secondary explant with a rooting medium comprising a modified MS basal culture medium, IAA, IBA, and NAA. The methods and compositions of the invention are capable of inducing extraordinarily rapid in vitro propagation of Swertia chirata . The methods and compositions of the disclosure may be useful for conservation of this threatened species as well as producing bulk quantities, e.g. gram, kilogram or more, of plant material for medicinal |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110521599-A |
priorityDate | 2001-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.