http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004265855-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_323dc7487353129cb7b4a68f7bf445e8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00 |
| filingDate | 2004-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f621c9327597b38cbb60aeb002ed50b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b020f2a6aa69592c491fa0177384df0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5536ddaa1d5b8f89262f06e17f7fd8f8 |
| publicationDate | 2004-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004265855-A1 |
| titleOfInvention | High-throughput DNA-isolation and transfection for analysing the function of genes or genetic products |
| abstract | The present invention relates to a method for screening a collection of nucleic acid molecules for a desired property of the nucleic acid or of a (poly)peptide encoded thereof, comprising the steps (a) automated picking of the cell collection containing the collection of nucleic acid molecules with a first robot; (b) automated lysis of the cells with a second robot; (c) automated separation of the cell DNA from the cell debris with a second robot; (d) optionally automated separation of endotoxins from the DNA with the second robot if the cells are bacteria; (e) automated transfection of the cells with the DNA obtained in step (c) or, if the cells are bacteria, with the DNA obtained in step (d) with a third robot; and (f) automated screening for the desired property with a fourth robot. Moreover, the invention relates to methods for the enhancement of the binding properties of the (poly)peptide identified by the of the screening method of the invention or encoded by the DNA identified and isolated and a method for the production of a pharmaceutical composition on the basis of (poly)peptides which can be obtained with the method of the invention and moreover the formulation of the substance obtained with a pharmaceutically acceptable carrier or dilutent. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2049690-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7590680-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10921338-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2013522578-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10132821-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9335336-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2049690-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11614454-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008005255-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10877057-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8496801-B2 |
| priorityDate | 2001-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.