abstract |
The object of the present invention is to provide a novel process for producing isomaltose and uses thereof and is solved by providing a process for producing isomaltose characterized in that it comprises the steps of allowing α-isomaltosylglucosaccharide-forming enzyme, in the presence or the absence of α-isomaltosyl-transferring enzyme, to act on saccharides, which have a glucose polymerization degree of at least two and α-1,4 glucosidic linkage as a linkage at the non-reducing end, to form α-isomaltosylglucosaccharides, which have a glucose polymerization degree of at least three, α-1,6 glucosidic linkage as a linkage at the non-reducing end, and α-1,4 glucosidic linkage as a linkage other than the non-reducing end, and/or to form cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→}; allowing isomaltose-releasing enzyme to act on the formed saccharides to release isomaltose; and collecting the released isomaltose; and uses thereof. |