http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004241831-A1

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filingDate 2004-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5cb5b9f8397a3331d346ac223c1932d
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publicationDate 2004-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004241831-A1
titleOfInvention tdcBC/pckA gene-inactivated microorganism and method of producing L-threonine using the same
abstract The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
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priorityDate 2003-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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