http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004224910-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P33-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-705 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K45-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P33-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 |
| filingDate | 2001-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1982e263f985e65f20abb5caf869762f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbc4c346215cf62ccdc62c638ac08671 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00b50b3becca15de0845f82d19f88129 |
| publicationDate | 2004-11-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004224910-A1 |
| titleOfInvention | Recombinant nematode nicotinic receptor and uses |
| abstract | We describe the molecular and functional characterization of the C. elegans unc-63 gene, a levamisole resistance locus on chromosome I, which encodes a nicotinic acetylcholine receptor (nAChR) α subunit. The derived amino acid sequence of UNC-63 most closely resembles that of UNC-38, the product of a separate levamisole resistance locus. Using a gfp::unc-63 fusion construct, expression has been detected in muscles (body wall, vulval) and motorneurons of C. elegans . Nuclear injection into Xenopus laevis oocytes of unc-63 cDNA together with lev-1 and unc-29 results in the expression for the first time of a robust functional C. elegans heteromeric nAChR. The EC 50 for ACh of the expressed receptor (20 μM) resembles that of native nematode muscle nAChRs. Nicotine and the anthelmintic drug levamisole are agonists and mecamylamine is an antagonist of this expressed receptor. When unc-38 cDNA is co-injected with cDNAs encoding unc-63, lev-1 and unc-29 much smaller amplitude agonist-activated currents are observed. The first robust functional recombinant heteromeric nAChR composed only of invertebrate subunits has therefore been characterized and shown to contain a pesticide binding site. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10345292-B2 |
| priorityDate | 1999-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 235.