| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cc46b81442e227275241082192c8583
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b8a26ec1cfd32e832f5950b031ffe937 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate |
2003-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d5c34ff03f11e5fe1c200f74684f854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d28263341ee97ff013e775d59ec65bf0 |
| publicationDate |
2004-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2004209272-A1 |
| titleOfInvention |
Orientation-directed construction of plasmids |
| abstract |
A method for orientation-directed construction of a construct including at least two nucleic acid segments of interest. Products are provided having phosphorylated blunt ends derived from the nucleic acid segments of interest. Separate ligation reactions are performed wherein in each reaction the phosphorylated blunt-ended products of two different segments obtained in the previous step are ligated to create a combined ligated sequence. PCR amplification reaction is performed using the combined ligated sequence obtained in the previous step as a template and specific enrichment primers direct the PCR amplification towards the desired orientation. Each combined ligated sequence is amplified in a separate reaction creating a combined product having phosphorylated blunt ends. The combined product is isolated and purified to have the enriched desired orientation. Self-ligation of the isolated purified combined product is performed to create a circular double-stranded DNA construct containing the desired segments properly aligned and operably linked. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450595-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9725760-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9416387-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9551027-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10745745-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9916428-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11254960-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10522245-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11603558-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014145298-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10017809-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10131939-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11649487-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10283217-B2 |
| priorityDate |
2001-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |