| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6424
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 |
| filingDate |
2002-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e65ec14b736d755e11d8bb6fd9c709ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0369a2c76b201c9bf9af0777440e5cd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d24cd914ae619d6a74cfda37762eec50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae4703d6bdd87589cbb201f57d96132c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_920e53dcf9d0cd8dd7083259f66a5d80 |
| publicationDate |
2004-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2004197858-A1 |
| titleOfInvention |
Process for producing human thrombin by gene modification technique |
| abstract |
A genetic recombinant human thrombin is provided. Human thrombin is efficiently prepared by the genetic engineering technique comprising the steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a gene encoding human prethrombin is incorporated to the downstream of a promoter so as to produce and accumulate prethrombin in culture supernatant and recovering the produced human prethrombin; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human thrombin; and (3) purifying the solution obtained after the above activation process to obtain purified human thrombin. The present invention allows for provision of human thrombin in a large scale in a safe and economical manner due to exclusion of blood-derived components. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008312127-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8697440-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8304224-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2778225-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8206967-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004219140-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7989193-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009047273-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005164367-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9447402-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2008275828-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7842477-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011092429-A1 |
| priorityDate |
2001-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |