| abstract |
The invention relates to a method for distinguishing at least two target bio-organic molecules with dyes selected from a pool of at least two dyes, the method comprising: (a) providing a first set of at least two probes, wherein each probe recognizes a target bio-organic molecule in a first set of target bio-organic molecules, and wherein each probe is distinctly-labelled with primary labels that are distinct from one another due to the presence of dyes in distinct ratios; (b) providing a second set of probes distinctly-labelled with said primary labels described in step (a), wherein each probe in said second probe set recognizes a target bio-organic molecule in a second set of target bio-organic molecules; wherein each probe in said first or second probe set is further labelled with the same first binary label, wherein said first binary label is distinct from said primary labels; and (c) contacting said at least two target bio-organic molecules with said probe sets, wherein said target bio-organic molecules are distinguished. |