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filingDate 2002-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2004-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004152099-A1
titleOfInvention Screening method for attenuating or virulence defective microbial cells
abstract This invention relates to a novel method for the genome saturating identification of nucleic acid sequences which are essential for infectivity and/or intracellular survival and/or propagation in permissive eukaryotic host cells, in particular microbial sequences. Further, a method for the identification of attenuated microorganisms and novel antimicrobial compounds using the identified essential nucleic acids and proteins encoded thereby is provided.
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