http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004137454-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2002-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd3a1f1d31d52ba2162c44955aff25be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84a4ca79cf9295bfc8b8425fd0889fb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d4b8a3050d0f87b26d6e7bb6bdb0e70 |
| publicationDate | 2004-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004137454-A1 |
| titleOfInvention | Assay for drug-induced recoding |
| abstract | A tissue culture assay for measuring drug-induced recoding in regulating cellular polyamine levels is described. A DNA construct containing the renilla luciferase gene separated by sort cloning site from the firefly luciferase gene, both under the control of a single upstream SV40 promoter is provided. The cloning site contains the portion of antizyme gene known to contain the mRNA signals for polyamine stimulated frameshifting with the downstream firefly gene in the +1 position relative to the upstream renilla gene. A control construct is also produced with the genes in the same reading frame. Frameshifting efficiencies can be determined by comparing the ratio or firefly to renilla luciferase activity in parallel cell cultures. |
| priorityDate | 2002-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 453.