| abstract |
The present invention provides a method of producing an evolved clostridial toxin light chain having altered protease specificity by (a) generating a population, each member of which contains a clostridial toxin light chain variant or functional fragment thereof; (b) assaying the population for protease activity towards a selected clostridial toxin-resistant target protein, where increased protease activity is indicative of an evolved clostridial toxin light chain; and (c) isolating from the population one or more members, which contain an evolved clostridial toxin light chain or functional fragment thereof. Also provided herein are compositions which contain an evolved clostridial toxin light chain or functional fragment thereof having altered protease specificity. |