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filingDate 2003-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_038581ba3cd3294e3cf5a06acfb883fe
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publicationDate 2004-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004110192-A1
titleOfInvention Class I sequence based typing of HLA-A, -B, and -C alleles by direct DNA sequencing
abstract There is provided a method for directly typing or sequencing HLA-A, -B, or -C alleles from a tissue sample wherein exons 2 and 3 of the HLA-A, -B, or -C alleles from the sample are amplified together in a locus specific manner and then separated out and individually amplified in a locus specific manner. After the two amplifications, the amplified exons are directly sequenced, the sequences are recombined, and a comparison is made between the derived HLA allele sequence and an HLA allele database, thereby giving an exact HLA-A, -B, or -C type for the sample being tested.
priorityDate 1997-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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