| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A21D8-042
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-386
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-38627 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L7-104
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A21D8-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C11D3-386 |
| filingDate |
2002-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfbf407bcc1b760b2473aefcda10fc07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ced43a101fa3b634e62300ce44a07173
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b35bfa55a62ddd8b8ef5663c1b60cbcb |
| publicationDate |
2004-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2004101928-A1 |
| titleOfInvention |
Lipolytic enzyme genes |
| abstract |
The inventors have isolated novel genes with a high homology to the T. lanuginosus lipase gene and are thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The expressed proteins may further be screened to identify lipolytic enzymes with improved properties. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity). |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8956853-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8460905-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8541211-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008182322-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009069587-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8241876-B2 |
| priorityDate |
2001-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |