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filingDate 2002-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78b1d3730596ce80c4b812916b1150a7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d99b9330021e09fdb184eaff3d4b166
publicationDate 2004-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004101925-A1
titleOfInvention Method and test kit for the detection of insecticide resistance in insect vectors
abstract A method for checking for elevated activity of mixed-function oxidases using a single insect, which comprises homogenizing a single insect in an acid pH buffer, preferably by grinding it in a small tube, such as an Eppendorf tube, with a pestle, adding a buffered tetramethylbenzidine solution as substrate, adding hydrogen peroxide as oxidant, and checking the blue coloration, the intensity of which indicates the degree of elevated mixed function oxidase activity. The invention also provides a test kit for use in the method which comprises separate containers of: n I. an acid pH buffer, preferably pH4 buffer n II. tetramethylbenzidine n III. a solvent for tetramethylbenzidine, preferably methanol, and n IV. hydrogen peroxide.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7223555-B2
priorityDate 2002-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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