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filingDate 2001-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a55734280260160d5f288f84c1841b25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b202f679e6940eeaa651bc8387a3f4bd
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publicationDate 2004-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2004091989-A1
titleOfInvention L-glutamate oxidase
abstract The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: n (A) action: catalyzing the following reaction: n L -glutamic acid+O 2 +H 2 O→α-ketoglutaric acid+H 2 O 2 +NH 3 ; n (B) substrate specificity: being specific to L-glutamic acid; n (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000; n (D) optimum pH: around pH 6.0 to 8.5; n (E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and n (F) coenzyme: flavin adenine dinucleotide (FAD).
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priorityDate 2000-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 48.