http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004078844-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8216 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2001-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_114a2d07012be1541ac845c427e6defe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_91c1bcdf1152c30de23186e82c473746 |
| publicationDate | 2004-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004078844-A1 |
| titleOfInvention | Methods and means for gene silencing |
| abstract | Provided are insolated DNA vectors which may be based on Agrobacterium binary vectors and which comprise: (a) a transfer nucleotide sequence comprising (i) a plant active promoter, operably linked to (ii) a recombinant tobacco rattle virus (TRV) nucleic acid which may corresponds to all or part of TRV RNA 1 and which includes: a sequence encoding a TRV trans acting factor, and cis acting elements, which confer on the TRV nucleic acid transcript the ability to replicate in the cytoplasm of a plant cell; and a heterologous nucleotide sequence which is foreign to said virus (which may be a cloning site, or a targeting sequence which is capable of down-regulating expression of a target gene); (b) border sequences which permit the transfer of the transfer nucleotide sequence into a plant cell genome. Preferred vectors include pBTAΔAMPΔ16K (SEQ ID NO: 3) or pBTAΔMP (SEQ ID NO: 2). Also provided are related materials and methods of use of such vectors e.g. to produce a cytoplasmically-replicating RNA which can be used to silence target genes in plants. |
| priorityDate | 2000-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 387.