http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004053341-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2002-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_582d3500df1e8a6086f8edabda2eac68 |
| publicationDate | 2004-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004053341-A1 |
| titleOfInvention | Method for treating histological and biological samples |
| abstract | A method of processing histological and biological specimens includes the sequential immersion of specimens into an aqueous solution of fixing agent, into at least three dehydrating liquids containing water and into at least three waterless dehydrating liquids. A chemical marker is added to the water contained in the aqueous solution of fixing agent, and a chemical indicator, forming a colored composition with the chemical marker, is added to the waterless dehydrating liquid. On appearance of a visually perceptible coloring in the waterless dehydrating liquid, that liquid is renewed. Ethyl and isopropyl alcohols or mixtures based thereon are used as dehydrating liquids. Transition metal salts having an oxidation rate of 2+ are used as chemical markers, such as salts of iron, cobalt, nickel, copper and zinc in the amount of 2-40 g (on the basis of metal) per liter of water. 1,10-phenanthroline, diphenylthiocarbazone, 1-nitroso-2-naphthol, sodium diethyldithiocarbamate, 8-oxyquinoline, 1-(2-pyridilazo)-2-naphthol, 4-(2-pyridilazo)-resorcinol or 2-carboxy-2′-hydroxy-5′-sulfoformazylbenzol are used as indicators in the amount of 0.001-0.4 g per liter of alcohol, or potassium or sodium thiocyanate in the amount of 5-10 g per liter of alcohol. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102841006-A |
| priorityDate | 2001-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.