http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004053230-A1
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2001-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d572b4a75c43fe10f156ac1d9e2362bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a73b11591fddac015e7322ffc8521cb0 |
publicationDate | 2004-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2004053230-A1 |
titleOfInvention | Real time quantitative pcr with intercalating dye for single and multiplex target dna |
abstract | The PCR-based, dsDNA quantification method monitors the fluorescence of a target, whose melting characteristics is predetermined, during each amplification cycle at selected time-points. Fluorescence is measured immediately after the annealing phase (F E at T E ), immediately below (F MS at T MS ) and above (F ME at T ME ) the melting of the target/amplicon. A change in slope from a baseline slope (S B =−(F MS −F E )/(T MS −T E )) to a melting phase slope (S M =−(F ME −F MS )/(T ME −T MS ) indicates a specific amplification. The number of amplification cycles (C T ) it takes for the quantity (S M −S B ) to become greater than zero correlates with the starting concentration of the target (C). The concentration of the target in a sample is determined by comparing the value of C T for the sample with a standard curve. By selecting targets with distinguishable melting curve characteristics, multiple targets can be simultaneously detected. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014242594-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004014119-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8039216-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010291583-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7785786-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108753932-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3901243-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9791372-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9556427-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2880184-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107164559-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10770170-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007172836-A1 |
priorityDate | 2001-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 715.