| abstract |
A biologically active fraction (BAF) is presented containing mainly S-lipopolysaccharide (LPS) from gram-negative bacteria producing endotoxic LPSs. These fractions are characterized in that in the lipid A of S-LPS the mole ratio of D-glucosamine and β-hydroxy acids selected from the group comprising β-hydroxydecanoic, β-hydroxydodecanoic, β-hydroxytetradecanoic, β-hydroxyhexadecanoic, is approximately 2:1-4, and the mole ratio of D-glucosamine and higher fatty acids, connected both by amide and ester links, in the lipid A of S-LPS is approximately 2:3-7. A method of isolating BAF, containing mainly S-LPS from gram-negative bacteria producing endotoxic S-LPSs, is also presented. n The obtained BAFs have pyrogenicity at the level of commercial polysaccharide vaccines and low endotoxicity, have high immunogenicity, which makes it possible to use them as vaccines for mammals, including humans. They are an inducer of cytokines and also may be considered to be a prophylactic tolerogenic anti-shock preparation. |