| abstract |
The present invention deals with a method for the generation of a dynamic combinatorial library of ligands for a given target, capable of binding at least two functionalities, which method comprises the following steps: (i) selecting a set of n molecules carrying functionalities which may bind to the target, which molecules are capable of reversibly binding to each other under formation of an entity, n being an integer ≧ 1 : (ii) mixing together said set of said n molecules: (iii) subjecting the mixture to conditions allowing a reversible bond formation and cleavage under formation of entities carrying different combinations of functionalities, until equilibrium is reached: (iv) optionally transforming the said entities into ligands: (v) repeating steps (i) to (iv) n-times, each time a different molecule of said set of n molecules being omitted: (vi) assessing the ability of each of the mixtures obtained to bind to a given target. |