Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24322 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24334 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-187 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
filingDate |
2003-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c8c1cb81bde66761206af3d5ec982d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7dfc9976ee77eca335e293092e4b6ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99ccdac67ceb12f69e58899880637478 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f68c4a34b19e1a5b63bb3799040e6b4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd2dfa1ae4d1cd2ad917cd2a12e6c663 |
publicationDate |
2004-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2004028701-A1 |
titleOfInvention |
E2 subunit vaccine comprising recombinant pestivirus E2 protein |
abstract |
The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×10 5 to 5×10 6 cells/ml with an m.o.i of <0.01. The invention also provides a method to produce recombinant pestivirus E2 or E m9 protein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 μg/ml. The invention also provides a method of producing recombinant FSH, α-units and/or β-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 μ/ml. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007098717-A2 |
priorityDate |
1997-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |