| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56911
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-77
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate |
2003-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a4ad9521cf481ce100d9056a6fa63de
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b713003a944228748d5133fd01fbb6f6 |
| publicationDate |
2004-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2004014154-A1 |
| titleOfInvention |
Methods and apparatus for assays of bacterial spores |
| abstract |
A sample of unknown bacterial spores is added to a test strip. The sample of unknown bacterial spores is drawn to a first sample region on the test strip by capillary action. Species-specific antibodies are bound to the sample when the unknown bacterial spores match the species-specific antibodies, otherwise the sample is left unbound. DPA is released from the bacterial spores in the bound sample. The terbium ions are combined with the DPA to form a Tb-DPA complex. The combined terbium ions and DPA are excited to generate a luminescence characteristic of the combined terbium ions and DPA to detect the bacterial spores. A live/dead assay is performed by a release of the DPA for live spores and a release of DPA for all spores. The detection concentrations are compared to determine the fraction of live spores. Lifetime-gated measurements of bacterial spores to eliminate any fluorescence background from organic chromophores comprise labeling the bacterial spore contents with a long-lifetime lumophore and detecting the luminescence after a waiting period. Unattended monitoring of bacterial spores in the air comprises the steps of collecting bacterial spores carried in the air and repeatedly performing the Tb-DPA detection steps above. The invention is also apparatus for performing the various methods disclosed above. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8362435-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005136508-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003138876-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8697374-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7608419-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010068756-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010075371-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007117175-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9816126-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7611862-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9850516-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7306930-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010078465-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010078465-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7563615-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006292664-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015307913-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9469866-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007031916-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8173359-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005221418-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2013130272-A1 |
| priorityDate |
2002-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |