http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004009483-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2002-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_857d2df0aac56444603a0f6eb22d239f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f75030dc3dd02f0d07b46a431dd7486e |
| publicationDate | 2004-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2004009483-A1 |
| titleOfInvention | Method of linear mRNA amplification using total RNA |
| abstract | Methods for linearly amplifying mRNA using total RNA to produce antisense RNA are provided. In the subject methods, mRNA is converted to double-stranded cDNA using a promoter-primer having a poly-dT primer site linked to a promoter sequence so that the resulting double-stranded cDNA is recognized by an RNA polymerase. A feature of the subject methods is that the source of mRNA employed in this conversion step is total RNA and the conversion step lasts at least about 4 hours. The resultant double-stranded cDNA is then transcribed into antisense RNA. The subject methods find use a variety of different applications in which the preparation of linearly amplified amounts of antisense RNA is desired. Also provided are kits for practicing the subject methods. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006183701-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008003574-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008003575-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006211004-A1 |
| priorityDate | 2002-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 382.