| abstract |
The invention features a method of identifying a nucleic acid molecule capable of post-transcriptional gene silencing by (a) affixing a plurality nucleic acid molecules onto a surface in discrete, defined locations; (b) contacting eukaryotic cells with the affixed nucleic acid molecules under appropriate conditions for entry of the nucleic acid molecules into the cells, whereby said nucleic acid molecules are introduced into the cells in the location in which they were affixed; and (c) determining the ability of the nucleic acid molecules to post-transcriptionally silence expression of a gene in the cells, wherein post-transcriptional gene silencing at a discrete, defined location identifies the nucleic acid molecules affixed at that location as being capable of post-transcriptional gene silencing. |