http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003219773-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9a652d3fb63e6921579775d6eb0a3fa |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2003-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53adaad0bf1f3ff623cfc1282e469a13 |
publicationDate | 2003-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2003219773-A1 |
titleOfInvention | Joining DNA sequences using topoisomerase I |
abstract | Restriction Independent Cloning Events (RICE) are made by generating 5′ overhangs (sticky ends). The polynucleotides to be joined are reacted with a DNA polymerase, having 3′ to 5′ exonuclease activity and 5′ to 3′ polymerizing activity, less than all of the dNTPs, a kinase (optional) and a ligase. The complementary 5′ overhangs anneal and ligate. Further disclosed is a method of joining double stranded polynucleotides that includes joining a first DNA sequence and a second DNA sequence using topoisomerase I to form a first product, joining the second DNA sequence and a third DNA sequence using topoisomerase I to form a second product, and combining the first product and the second product in PCR reaction to generate a third product. |
priorityDate | 2002-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.