| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 |
| filingDate |
2001-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b02a43c1950faea2eff49bb58ffafd5e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ac1a3fd86a82a54a356600fc1fa2704 |
| publicationDate |
2003-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003166054-A1 |
| titleOfInvention |
Enhanced in vitro protein synthesis |
| abstract |
Poly G tails prolong mRNA chemical and functional half life in E. coli cell extracts and dramatically increased RNA-dependent protein synthesis in vitro. The effect of polyguanylation on mRNA functional half life, as measured by the ability of CAT transcripts to produce biochemically-active protein in vitro, was four- to six-fold greater than the effect on chemical half life. Addition of a few nucleotides 5′ to the bacteriophage T7 promoter markedly enhanced transcription of linear PCR-generated DNA molecules by T7 RNA polymerase. Collectively a novel approach is provided for efficient in vitro protein synthesis that bypasses the need for cloned DNA templates to obtain the products of translational open reading frames. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011117597-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009010907-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11535876-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284547-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8445228-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8486865-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113801877-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9677068-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9388445-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010120625-A1 |
| priorityDate |
2000-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |