| abstract |
Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve n a) expressing a diversified population of nucleotide sequences encoding a polypeptide of interest, n b) screening the polypeptides expressed in step a) for function, immunogenicity and/or stability, n c) selecting functional polypeptides having altered immunogenicity and/or increased stability, e.g. functional in vivo half-life as compared to the polypeptide of interest, and n d) optionally subjecting the nucleotide sequence encoding the polypeptide selected in step n c) to one or more repeated cycles of steps a)-c). In a further step the expressed polypeptides of step a) or c) can be conjugated to at least one non-polypeptide moiety. |