| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2003-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3f247d3b45c0e27f08aa6ef6cbc69bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f872409d1824a9ff442d4a195deb46b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc6939dc18c3279fba7729118749130b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_995a058f5e1742aa8502322d8ebb2e1a |
| publicationDate |
2003-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003152984-A1 |
| titleOfInvention |
Method for the manufacture of DNA |
| abstract |
The present invention relates to a method for manufacturing DNA comprising the steps of preparing n single stranded base-DNA-oligonucleotides which form immediately consecutive parts of the nucleotide sequence of the DNA being manufactured, in which the second to the n-th base-DNA-oligonucleotide is phosphorylated at the 5′ end and n is at least 2; preparing at least (n−1) single stranded joint-DNA-oligonucleotides capable of functioning as ligation templates for the base-DNA-oligonucleotides; contacting the base-DNA-oligonucleotides with the joint-DNA-oligonucleotides; subjecting the resultant product DNA-hybrid to a ligation reaction; and finally subjecting the resultant reaction product to an exonuclease reaction, in which the DNA strand of the reaction product formed by ligated base-DNA-oligonucleotides includes at least two cap-structures. Further the invention relates to DNA obtained by the method and a kit for carrying out the method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3763818-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3964285-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007238125-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112175940-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/DE-102010056289-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10563240-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020001783-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10519439-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012084923-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017062343-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021178809-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013049227-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007275006-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107760742-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020212391-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3557262-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008118915-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11046953-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7727745-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10407676-B2 |
| priorityDate |
2002-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |