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filingDate 2002-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c77b49733f4e9cd8672fe7c31f778b9b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75d097a6c407b7943223e546fe97726f
publicationDate 2003-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2003143703-A1
titleOfInvention Recombinant microorganism expressing polyhydroxyalkanoate biosynthesis enzyme and intracellular PHA depolymerase
abstract The present invention provides recombinant plasmids containing a gene coding for polyhydroxyalkanoate (PHA) biosynthesis enzyme and a gene coding for intracellular PHA depolymerase, and a process for preparing (R)-hydroxycarboxylic acid employing the same. In accordance with the present invention, optically pure (R)-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give (R)-hydroxycarboxylic acid. The process for preparing (R)-hydroxycarboxylic acid of the invention can be successfully employed in large-scale continuous process with a high productivity by carrying out PHA synthesis and degradation in a simultaneous manner, while enjoying the benefits of simple procedures for harvesting cells and disposing the cell wastes.
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type http://data.epo.org/linked-data/def/patent/Publication

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