| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0063
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00626
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00621
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-0061
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00725
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54333
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54366 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2002-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b6fbfd9f0944fe2650ce6243f2468e6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6c497791156227221767b525ef61942
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aade55ffb168936e106705037ed88bb9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_070f2bb6b7235a0ef7d0a2f142327cac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c617b5b09e4df5c75a82bf2a831bcda |
| publicationDate |
2003-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003143576-A1 |
| titleOfInvention |
Method and device for integrated protein expression, purification and detection |
| abstract |
A method for presenting a target protein or an array of target proteins for analysis is disclosed. The method includes the steps of forming a coding sequence; placing the coding sequence and protein-synthesis components capable of expressing the target protein under selected protein-synthesis conditions in a well on a substrate, the well having a surface which has been functionalized with the second coil-forming peptide; expressing said coding sequence under such conditions, wherein the target protein so synthesized binds to the well through coil-coil heterodimer formation, and is thus presented for analysis in the well in captured form; and washing said well to remove unbound components. The coding sequence includes a first nucleic acid sequence which encodes a first coil-forming peptide having a selected charge and being capable of interacting with a second, oppositely charged coil-forming peptide to form a stable α-helical coiled-coil heterodimer; and a second nucleic acid sequence encoding the target protein. Also disclosed are kits for use in practicing the method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009192048-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7889347-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009312192-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7687437-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010105567-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8094315-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7463358-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007128455-A1 |
| priorityDate |
2001-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |