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filingDate 2001-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab2744ab6d73f4c356cab78bad41387f
publicationDate 2003-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2003129594-A1
titleOfInvention Genes and the physics of the DNA double-helix. formulation of a physics-based gene identification (PBGI) method: ab initio identification of genes in eukaryotic genomes
abstract Method for the identification of genes and genetic signals based on the structural properties of DNA double-helix comprising the following steps: n A—using the classical physical model of helix-coil transitions, n B—calculating stability curves (probabilities of opening of the DNA double-helix, along a given sequence) by algorithmic methods), n C—determining the disruption in the linear DNA for different temperatures, n D—analyzing the stability curves for the detection of genetic signals (disruption of the double-helix) or the identification of coding regions (simple genes or exons in split genes as regions of high thermal stability), and optionally, n E—based on the structural informations, performing classical sequence analysis (donor/acceptor sites, start and codon stops, in correspondence with the frontiers identified in the stability curves and open reading frames analyses) for completing the identification of genes.
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