| abstract |
Filtration-based microarray chips are provided. The microarray chips comprise a random oriented microporous filtration substrate of charged cellulose esters, such as nitrocellulose, and a plurality of different analyte-specific capture molecules attached to the substrate in a microarray. The capture molecules can be proteins, antigens, monoclonal or polyclonal antibodies, or apatomers. In particular, apparati and methods for the detection of a subject analyte comprising multiple stacked filtration-based microarray chips are also provided. The filtration-based microarray chips permits a sensitive, specific, fast and high throughput assay that can be used to detect analytes associated with a disease. |