| abstract |
The invention relates to nucleic acid expression cassettes and vectors comprising these expression cassettes, where two or more transgenes can be expressed. In general, the expression cassettes orient at least two of the transgenes in opposite directions with respect to their reading frames. In one aspect, vectors comprising these expression cassettes advantageous can be used to provide relatively equal levels of expression of each of the two or more transgenes. In particular examples, the vectors can be used in methods to treat disease by allowing multiple therapeutic polypeptides to be expressed in the same cell. |