| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2408
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2414
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04 |
| filingDate |
2001-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43393007e03ab2245085101944fbd39e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_804db1c47c0880389ad539cfac6bc63c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95831d58e5f7abbee7a2c45c2905f474 |
| publicationDate |
2003-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003100749-A1 |
| titleOfInvention |
Methods and compositions for synthesis of oligosaccharides using mutant glycosidase enzymes |
| abstract |
Mutant glycosidase enzymes are formed in which the normal nucleophilic amino acid within the active site has been changed to a nonnucleophilic amino acid. These enzymes cannot hydrolyze disaccharide products, but which can still form them. Using this enzyme, oligosaccharides are synthesized by preparing a mixture of an &agr;glycosyl fluoride and a glycoside acceptor molecule; enzymatically coupling the &agr.glycosyl fluoride to the glycoside acceptor molecule to form a glycosyl glycoside product using the mutant glycosidase enzyme; and recovering the glycosyl glycoside product. Particular enzymes include a mutant form of Agrobacterium &bgr.Glucosidase in which the normal glutamic acid residue at position 358 is replaced with an alanine residue. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1690876-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7795240-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1690876-A1 |
| priorityDate |
1995-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |