| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00387
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00659
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S436-80 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-312
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B50-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16H10-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-00871
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-025 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16H10-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-31
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F17-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 |
| filingDate |
2002-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1772806c19620eff2272d37f61d220bc |
| publicationDate |
2003-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2003087286-A1 |
| titleOfInvention |
Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles |
| abstract |
The present invention relates to a method to isolate fully methylated unamplified eukaryotic DNA made of hundreds-of-thousand to billions of base pairs from a lysate. The eukaryotic tissue samples are digested with protolytic enzymes in buffer releasing cellular contents. Magnetically responsive functionalized solid particles are added to the lysate. Polyethylene Glycol and a high salt concentration are added to the mixture to disrupt the hydrogen bonding in the lysate. The eukaryotic genomic DNA binds to the functionalized microparticles and is washed a number of time with an alcohol mixture to remove and denature proteins. The genomic DNA is the eluted off the magnetically responsive functionalized solid particles and used in downstream reactions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002177137-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006150155-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006014192-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005221370-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005170423-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003207289-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005272085-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7011943-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006014186-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003082605-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6977178-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006001831-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7282361-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005266494-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2455780-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005239125-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7494817-B2 |
| priorityDate |
2000-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |