Predicate |
Object |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2502-13 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 |
filingDate |
2002-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b274cb9ebce945e71fffc506f8932019 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e89e85ccad5be477c489bc6cc8670359 |
publicationDate |
2003-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2003077822-A1 |
titleOfInvention |
Enzyme solution for culturing primate embryonic stem cells and method of culturing using it |
abstract |
An enzyme solution for subculturing primate embryonic stem (ES) cells and a method of culturing primate ES cells is described herein. The enzyme solution comprises trypsin, calcium chloride and a serum substitute. The culturing method comprises the step of culturing primate ES cells in a solution comprising trypsin and calcium chloride, and optionally a serum substitute. The solution and method of this invention enable one to stably maintain and propagate ES cells derived from a primate, such as monkey or human, for a long period in an undifferentiated state and with a normal karyotype. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011124763-A1 |
priorityDate |
2001-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |