Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9bceffddb90b9b3da8a772212961764b |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-16 |
filingDate |
2001-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2498073f88259b92775ca5106e1e763b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e8af9296fbc2607756a94aadf4f4f83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1933eea4fa7dc573383ef82295d7f32a |
publicationDate |
2003-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2003059872-A1 |
titleOfInvention |
Nucleic acids, expression vectors and host cells for making chimeric nucleic acids and methods for producing immobilized polypeptides |
abstract |
The present invention enables immobilization of a useful protein, for example, a glycosyltransferase, onto the surface of a yeast cell without deteriorating its enzyme activity. The present invention provides a fusion nucleic acid, expression vectors and host cells comprising these chimeric nucleic acids, and methods for making and using them. The chimeric nucleic acid is characterized by comprising a coding sequence encoding a useful protein bound to a yeast cell wall protein. In one aspect, it is downstream of a gene encoding the yeast cell wall protein. In one aspect, the host cell is a transformant yeast, which is transformed with the fusion gene expression vector that expressed an enzyme that is immobilized yeast cell wall. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2497478-A2 |
priorityDate |
2000-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |