http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003049637-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd15fea28fa5c3272dd3e41dcf3b0dd3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2001-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d1d4c59be1b2ff15db59728d529c554 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11483a6be88dab798274bce90de78c04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6725b631ca3ec379d8daf732e544201 |
| publicationDate | 2003-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2003049637-A1 |
| titleOfInvention | Process for preparation full-length cDNA and anchor used for the same |
| abstract | The present invention relates to a process for the preparation of full-length complementary DNA (cDNA). More particularly, the present invention is directed to a process for selective amplification of full-length cDNA, which comprises: i) a step for preparing a hybrid composed of a messenger RNA (mRNA) strand and a cDNA strand of which three (3) or four (4) deoxycitidinemono phosphate (dCMP) are combined at 3′ end, by treating mRNA with reverse transcriptase; separately from the above step, ii) a step for adenylating single strand anchor of which biotin or phosphate group is combined at 3′ end, and phosphate group is combined at 5′ end; iii) a step for ligating said adenylated single strand anchor to 3′ end of full-length cDNA strand of said cDNA/mRNA hybrid to select full-length cDNA/mRNA hybrid; and iv) a step for amplifying only the full-length cDNA/mRNA hybrid through polymerase chain reaction (PCR) which employs a primer of which base sequence is complementary to that of said anchor. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10538795-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014009413-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005164190-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8198052-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11000554-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2684954-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10220059-B2 |
| priorityDate | 2001-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID171548 http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID226399873 |
Total number of triples: 26.