http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003039989-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1086 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2001-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0907f957a1769ab28e25b4cda89aa3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd4f1225902bec42682b04aea75716c8 |
| publicationDate | 2003-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2003039989-A1 |
| titleOfInvention | Method for constructing an expression library and uses thereof |
| abstract | The present invention provides a method for constructing an expression library containing a gene that is toxic to bacteria, comprising the steps of: (a) isolating genomic DNA from a culture of cells of a first bacterium, wherein said genomic DNA contains a gene toxic to a second bacterium; (b) partially digesting said genomic DNA to produce genetic inserts, wherein at least one of the genetic inserts contains a gene to said second bacterium; (c) cloning said genetic inserts into a cloning vector; (d) ligating together, under suitable ligation conditions, said genetic inserts and said cloning vector, to produce ligation products; (e) transforming said ligation products directly into yeast; and (f) amplifying said ligation products in said yeast. Also provided are an expression library constructed in accordance with this method and a method for screening for a gene that is toxic to bacteria using said expression library. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109680344-A |
| priorityDate | 2001-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.