| abstract |
The present invention provides for a device and method for detecting genetic material. The device includes at least one hairpin sensor or, preferably two or more hairpin sensors, spatially and/or spectrally multiplexed on a conductive or semi-conductive substrate or particle. The at least one hairpin sensor includes a quenchable fluorescing agent bound to a hairpin loop assembly and the hairpin loop assembly includes a probe complementary to a nucleotide sequence of interest. The method includes providing at least one hairpin sensor, exposing the at least one hairpin sensor to a sample of interest, and detecting fluorescence produced by the quenchable fluorescing agent. The fluorescence indicates the binding of a target nucleotide sequence to the complementary probe of the hairpin loop assembly. |