| abstract |
The invention provides a method of generating and analyzing a urinary protein fragmentation profile, in terms of the size, and sequence of particular fragments derived from intact filtered proteins together with the position where enzymes scission occurs along the protein polypeptide chain is characteristic of the diseased state of the kidney. n With the recognition that filtered proteins are degraded during renal passage, the methods described in this application will be able to detect protein fragments derived from proteins generated by non-renal disease. Non-renal disease such as cancers may generate increased levels of proteins into the circulation. The urinary analysis of these filtered proteins would currently not detect the intact form of these proteins. Therefore, a method as described to detect and analyze fragments resulting from degradation during renal passage that will be able to detect the seriousness of the disease. |