http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002150983-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-78 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-80 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
| filingDate | 2001-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc9c007cd4c95048f745a995f2045ab5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2ba769b5d41818d62c77a7d01930b66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d4c4ce465f12706c128d03021fb58ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7954d643fc1a50aaffa10bb6f6dab093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbfc08e80b036f958851e260236159da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3a129f6bf798cd4e2c1cb44bd896f79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_352f4801bacae4d0c12459163b0fc3f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c4ad4ab4c6b71153cd6a6fa74fdd3f2 |
| publicationDate | 2002-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2002150983-A1 |
| titleOfInvention | Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same |
| abstract | Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained. Also, there is disclosed a pop-out technique in which a recombinant protein expression cassette is inserted into a MOX gene site of the mutant and is allowed to pop out therefrom, thereby utilizing the mutant as a host for general use in producing various proteins of interest |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/RU-2675471-C1 |
| priorityDate | 1999-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 142.