http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002137037-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2000-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68efddd7aa485eb01ae9cadc1a7af895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce48ece95de6f59eb67ce3569a33d1f3 |
| publicationDate | 2002-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2002137037-A1 |
| titleOfInvention | Method for DNA footprinting |
| abstract | The present invention describes a method for characterizing a DNA-protein complex that entails treating a DNA-protein complex with a DNA cleavage reagent. The bound protein blocks DNA cleavage in a region of the DNA molecule where the protein is bound, so that the DNA is only cleaved in regions that are not blocked by the bound protein. Ion pairing reverse phase high performance liquid chromatography is used to separate and detect the cleaved DNA, thereby identifying regions of the DNA where cleavage has occurred. The absence of cleavage events in a region of the DNA indicates that the protein bound to that region. In a preferred embodiment, the cleavage reagent is hydroxyl radical, the DNA is fluorescently labeled, and the chromatographic separation is carried out under conditions that are substantially free of multivalent cations that are free to interfere with polynucleotide separations. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103123343-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103123344-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2012070830-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8709717-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005011836-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2066814-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2066814-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7125492-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010108143-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010108143-A2 |
| priorityDate | 2000-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 510.