http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2002132256-A1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2001-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e56b07db5c9861f3ad562e374c6ab6c5 |
| publicationDate | 2002-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2002132256-A1 |
| titleOfInvention | Method for the detection and measurement of hapten-conjugated biological binding entities by western and dot-blot using anti-hapten antibodies |
| abstract | The invention is a procedure for measuring the binding of an entity (ligand) to a surface by using a hapten-conjugated version of the ligand (hapten-ligand). An excess of the hapten-ligand is presented to the binding surface and excess (unbound) hapten-ligand is washed off. Bound hapten-ligand is then solubilized (removed) and applied to a membrane support or separated by electrophoresis and applied to a membrane support. Standard amounts of hapten-ligand are similarly applied to the membrane. The membrane-bound hapten-ligand is detected by application of an enzyme-conjugated antibody to the hapten; or by application of an antibody to the hapten followed by application of an enzyme-conjugated antibody to the anti-hapten antibody. The resultant membrane-associated enzyme is detected and quantitated by the application of a color or light-producing substrate which reacts with the enzyme. A combination of the use of anti-hapten antibodies along with membrane-blotting technologies to assess hapten-ligand binding to surfaces is not found in the scientific or patent literature, particularly in regards to assessing protein binding to cell surfaces. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1382966-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004005627-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111337660-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7635680-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7645739-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005222030-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7635676-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7635678-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2009291086-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2006105952-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007015705-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113702341-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2008069823-A1 |
| priorityDate | 2000-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 188.